- "I AM NEGAN. Anyone else want a bullet?"
- —Regina after shooting a worker.[src]
Nothing is known about Regina's life prior to or as the outbreak began. It's possible she may have lived in the Virginia area before the outbreak.
At some point after the outbreak, Regina came into contact with the Saviors and joined them. Over time, she became a high ranking member and one of Negan's top lieutenants.
Regina makes her first appearance during the militia's attack on the Sanctuary. She exits the Sanctuary alongside Negan, Dwight, Simon, Eugene, and Gavin. When Rick calls the lieutenants to surrender by name, she introduces herself, as he didn't know who she was.
Regina and the others refuse to surrender, and once the militia opens fire on them, they proceed to flee back inside the Sanctuary. The fence is blown apart, and a herd surrounds the Sanctuary.
Regina is present for the meeting with Negan and his other lieutenants (Simon, Dwight, Eugene, and Gavin) at the Sanctuary. They discuss the joint rebellion of Alexandria, the Hilltop and the Kingdom. The meeting is interrupted when shots are fired by the militia, and Regina and the others exit to face the intruders. The events of "Mercy" then take place.
Following the herd and the loss of Negan, the lieutenants inside the Sanctuary debate what to do in their current predicament. Regina is the first to suggest that they must assume Negan is dead. Simon disagrees and questions if Regina is really Negan; she states that she is. She then proposes that they send a crew of forty workers outside to clear a path in the herd, but Eugene argues that the workers would be overcome in minutes. Regina explains that the workers would be a distraction while a small team snuck past the herd to warn the outposts. Eugene points out flaws in that plan as well, claiming it would cause unrest among the workers. They continue to disagree until Dwight becomes a voice of reason.
While the lieutenants are in another meeting, Laura enters to notify them that the workers are coming up the stairs. They all exit into the corridor and find it full of workers making demands. Someone asks where Negan is; when another worker draws a gun, Regina shoots him and says she is Negan. The workers and lieutenants alike kneel when they hear whistling down the hall, as they recognize the sound as Negan, who made it inside safe with Gabriel. The high-ranking Saviors reassemble again later, and Gary, Laura, and Arat inform the group that someone had taken guns from the armory to give to the workers.
Regina and other Saviors help open fire on the invading zombies when they break into the Sanctuary. She takes a position on the stairs as screaming workers flee to the upper floors, holding her fire once the pile of zombie corpses jam up the base of the stairwell. Afterwards, she enters Negan's office, along with other Saviors, pondering what else could be done to save the Sanctuary.
This list shows the victims Regina has killed:
Regina and Negan seem to have a good relationship and as one of Negan's top Lieutenants it is clear that Negan trusts her. Their relationship may have been strained due to Regina shooting one of the Savior workers commenting on why "you had to go and do that".
Regina and Dwight seem to have a steady relationship although Dwight is frustrated at her for failing to find a solution to their situation.
Both being outpost heads, Regina and Simon seem to have a steady relationship, although Simon briefly questions her loyalty.
Regina and Eugene seem to have an unsteady relationship as a result of Eugene contradicting her plan to escape the Sanctuary. Regina suggests sending Eugene out on his own. Regina also seems suspicious of Eugene's loyalty to the Saviors.
Regina and Gavin seem to have a stable relationship given that they are both among Negan's top Lieutenants.
- Regina appears to be the highest ranking female Savior and the only female leader of a Savior outpost; in this case the Shephard Office Plaza.
- She is the first named Native-American character to appear on The Walking Dead and second overall as the first one was Terminus Resident 1.